Molecular Cloning Protocol Simplified step-by-step molecular cloning protocol: 1. DNA Extraction: Begin with DNA extraction from the organism of interest. This DNA will contain the gene that you wish to clone. 2. PCR Amplification: Use Polymerase Chain Read More
Akta Pure Protein Purification Protocol Protein purification is a sequence of processes intended to isolate a singular type of protein from a complex mixture. ÄKTA pure is an adaptable and flexible chromatography system for preparative Read More
Nickel Column Protein Purification Protocol Nickel affinity column chromatography, also known as immobilized metal affinity chromatography (IMAC), is a common method for purifying recombinant proteins that are engineered to have a hexahistidine tag (6xHis tag) Read More
Cell Culture Protocol Cell culture is a common laboratory technique to grow and maintain cells under controlled conditions. Here is a general protocol: 1. Cell Culture Basics: – Always work in a sterile environment, such Read More
Polymerase Chain Reaction (PCR) Protocol Standard PCR protocol to amplify a target DNA sequence. Materials: 1. DNA Template 2. Forward and reverse primers 3. Nucleotides (dNTPs) 4. Taq polymerase 5. Buffer solution 6. An automated thermocycler Protocol: 1. Setup your PCR reaction: — Read More
Immunofluorescence Protocol (FFPE) A general protocol for performing immunofluorescence on formalin-fixed paraffin-embedded (FFPE) samples. Materials: 1. FFPE tissue sections/slides 2. Primary and secondary antibodies 3. Xylene 4. Ethanol in various concentrations 5. Citrate buffer (pH 6.0) or similar antigen retrieval Read More
Immunofluorescence Protocol General protocol for immunofluorescence. Please ensure you understand all steps and safety guidelines well before you proceed. Materials: 1. Primary and secondary antibodies 2. Phosphate-buffered saline (PBS) 3. Blocking solution 4. PFA fixative 5. Detergent (Triton X-100) 6. Glass Read More
Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) is a technique used to identify specific antigens in tissues by using specific antibodies that bind to them. Here’s a simple protocol: Materials: Steps: NOTE: This is a general Read More
SDS-PAGE Protocol The basic protocol for Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) is commonly used to separate proteins according to their molecular weight. Materials Needed: Steps: This is a generalized protocol; parameters can differ based Read More
Journal Impact Factor 2023-JCR Forecasts Latest Journal Impact Factor Forecasts in 2023 The iNature editorial department predicts the impact factors of some major biological and medical journals based on the latest Web of Science data ( for Read More
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