Mice Anesthetization Protocols Anesthetizing mice is a common practice in biomedical research for various purposes, including surgery, imaging studies, or the collection of tissues. Several methods are available, including injectable and inhalant anesthetics. 1. Injectable Anesthetics: Read More
SDS-PAGE Gel Preparation 1. Prepare the separation gel (10%). Mix in the following order: H2O 4.1 mL Acrylamide/bis (30% 37.5:1; Bio-Rad) 3.3 mL Tris-HCl (1.5 M, pH 8.8) 2.5 mL SDS, 10% 100 µL N,N,N′,N′-tetramethylethylene-diamine (TEMED) (Bio-Rad) 10 µL Ammonium Read More
Tamoxifen Preparation and Treatment in Mice for Genetic Recombinations Rationale: Tamoxifen is used to kick-start genetic recombinations in genetically engineered mouse models (GEMMs) and to activate genes through a cascade of events. Here is a Read More
DNA Quantification using Qubit Fluorometer The Qubit Fluorometer is a device from Thermo Fisher Scientific used to quantify DNA, RNA, and protein in samples by using a fluorescent dye that binds to these molecules. The Read More
DNA Quantification using Nanodrop spectrophotometer The nanodrop spectrophotometer is commonly used to quantify DNA concentration and assess purity. Here is a basic outline of the process: 1. Calibrate the machine: It’s usually done by using Read More
DNA Extraction Protocol in Detail Here is a widely accepted protocol for the extraction of DNA from cells, especially mammalian cells. This method is called the phenol-chloroform extraction method. Materials 1. Cell culture or tissue sample 2. Read More
RNA Extraction Protocol in Detail Let’s dive into details using a widely accepted protocol based on an RNA extraction kit (which utilizes the organic extraction method with TRIzol, which is a monophasic solution of Read More
Flow Cytometry Detailed Protocol Flow cytometry is a technique used for counting, examining, and sorting microscopic particles/cells suspended in a stream of fluid. Here is a detailed protocol for a typical immunofluorescence flow cytometry Read More
Enzymatic digestion protocol for single-cell suspension Creating a single-cell suspension through enzymatic digestion involves several steps in which tissues are broken down into individual cells. Here is a simple generic protocol that you could follow: Read More
ELISA Immunoassay Detailed Protocol Enzyme-linked immunosorbent Assay (ELISA) is a biochemical method used to detect the presence and concentration of antibodies or antigens in a sample. Materials needed: – ELISA Plates – Target antigen or antibody – Non-reactive Read More
530K+ and Counting..!
Click the ICON to Join
Now, it has been 5 years since it was launched in late July 2018 and is moving forward. Appreciate all the respected visitors! Please share this site with your friends to push science forward and improve human health.
