DNA Quantification using Qubit Fluorometer The Qubit Fluorometer is a device from Thermo Fisher Scientific used to quantify DNA, RNA, and protein in samples by using a fluorescent dye that binds to these molecules. The Read More
DNA Quantification using Nanodrop spectrophotometer The nanodrop spectrophotometer is commonly used to quantify DNA concentration and assess purity. Here is a basic outline of the process: 1. Calibrate the machine: It’s usually done by using Read More
DNA Extraction Protocol in Detail Here is a widely accepted protocol for the extraction of DNA from cells, especially mammalian cells. This method is called the phenol-chloroform extraction method. Materials 1. Cell culture or tissue sample 2. Read More
RNA Extraction Protocol in Detail Let’s dive into details using a widely accepted protocol based on an RNA extraction kit (which utilizes the organic extraction method with TRIzol, which is a monophasic solution of Read More
Scopus Journal List Introduction Scopus is one of the largest abstract and citation databases of peer-reviewed literature that covers fields like science, technology, medicine, social sciences, and more. It features smart tools for tracking, analyzing, Read More
Swiss Government Excellence Scholarships 2023-2024Read More
Flow Cytometry Detailed Protocol Flow cytometry is a technique used for counting, examining, and sorting microscopic particles/cells suspended in a stream of fluid. Here is a detailed protocol for a typical immunofluorescence flow cytometry Read More
Enzymatic digestion protocol for single-cell suspension Creating a single-cell suspension through enzymatic digestion involves several steps in which tissues are broken down into individual cells. Here is a simple generic protocol that you could follow: Read More
ELISA Immunoassay Detailed Protocol Enzyme-linked immunosorbent Assay (ELISA) is a biochemical method used to detect the presence and concentration of antibodies or antigens in a sample. Materials needed: – ELISA Plates – Target antigen or antibody – Non-reactive Read More
Protocol for Mouse spleen isolation and preparation of single-cell suspension Before carrying out any lab procedures, please consult your lab protocol and safety guidelines. Also, ensure all equipment used has been cleaned and sterilized according Read More
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