TCR Sequencing Protocol T-cell receptor (TCR) sequencing is a technique used to analyze or profile the repertoire of T-cell receptor sequences in a sample. This advanced immunogenetics method provides important insights about immune responses and Read More
Fluorescence Imaging Protocol Fluorescence imaging is a powerful tool often used in biological research to visualize cells and tissues. The protocol for fluorescence imaging can vary depending on the specific purpose of the study, the Read More
qRT-PCR Protocol qRT-PCR, also known as real-time reverse transcription PCR or quantitative reverse transcription PCR, is a laboratory technique used in molecular biology to amplify and simultaneously quantify a specific DNA target. It is often Read More
Transwell Cell Migration and Invasion Assay Transwell cell migration and invasion assays are widely used methodologies in biomedical research to study cells’ migratory and invasive capabilities. These assays are particularly relevant in the study of Read More
Ammonium Sulphate Fractionation of Antibodies (Crude Protein Purification) Abstract A “cut” with ammonium sulphate was the go-to procedure for isolating IgG and other blood proteins until Protein A (rabbit) and Protein G (rodent) were widely Read More
Molecular Cloning Protocol Simplified step-by-step molecular cloning protocol: 1. DNA Extraction: Begin with DNA extraction from the organism of interest. This DNA will contain the gene that you wish to clone. 2. PCR Amplification: Use Polymerase Chain Read More
Akta Pure Protein Purification Protocol Protein purification is a sequence of processes intended to isolate a singular type of protein from a complex mixture. ÄKTA pure is an adaptable and flexible chromatography system for preparative Read More
Nickel Column Protein Purification Protocol Nickel affinity column chromatography, also known as immobilized metal affinity chromatography (IMAC), is a common method for purifying recombinant proteins that are engineered to have a hexahistidine tag (6xHis tag) Read More
Cell Culture Protocol Cell culture is a common laboratory technique to grow and maintain cells under controlled conditions. Here is a general protocol: 1. Cell Culture Basics: – Always work in a sterile environment, such Read More
Polymerase Chain Reaction (PCR) Protocol Standard PCR protocol to amplify a target DNA sequence. Materials: 1. DNA Template 2. Forward and reverse primers 3. Nucleotides (dNTPs) 4. Taq polymerase 5. Buffer solution 6. An automated thermocycler Protocol: 1. Setup your PCR reaction: — Read More
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