Protocols

Mice Anesthetization Protocols Anesthetizing mice is a common practice in biomedical research for various purposes, including surgery, imaging studies, or the collection of tissues. Several...

SDS-PAGE Gel Preparation 1. Prepare the separation gel (10%). Mix in the following order: H2O4.1 mLAcrylamide/bis (30% 37.5:1; Bio-Rad)3.3 mLTris-HCl (1.5 M, pH 8.8)2.5 mLSDS, 10%100 µLN,N,N′,N′-tetramethylethylene-diamine (TEMED)...

Tamoxifen Preparation and Treatment in Mice for Genetic Recombinations Rationale: Tamoxifen is used to kick-start genetic recombinations in genetically engineered mouse models (GEMMs) and to activate...

DNA Quantification using Qubit Fluorometer The Qubit Fluorometer is a device from Thermo Fisher Scientific used to quantify DNA, RNA, and protein in samples by...

DNA Quantification using Nanodrop spectrophotometer The nanodrop spectrophotometer is commonly used to quantify DNA concentration and assess purity. Here is a basic outline of the...