Polymerase Chain Reaction (PCR) Protocol Standard PCR protocol to amplify a target DNA sequence. Materials: 1. DNA Template 2. Forward and reverse primers 3. Nucleotides (dNTPs) 4. Taq polymerase 5. Buffer solution 6. An automated thermocycler Protocol: 1. Setup your PCR reaction: — Read More
Immunofluorescence Protocol (FFPE) A general protocol for performing immunofluorescence on formalin-fixed paraffin-embedded (FFPE) samples. Materials: 1. FFPE tissue sections/slides 2. Primary and secondary antibodies 3. Xylene 4. Ethanol in various concentrations 5. Citrate buffer (pH 6.0) or similar antigen retrieval Read More
Immunofluorescence Protocol General protocol for immunofluorescence. Please ensure you understand all steps and safety guidelines well before you proceed. Materials: 1. Primary and secondary antibodies 2. Phosphate-buffered saline (PBS) 3. Blocking solution 4. PFA fixative 5. Detergent (Triton X-100) 6. Glass Read More
Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) is a technique used to identify specific antigens in tissues by using specific antibodies that bind to them. Here’s a simple protocol: Materials: Steps: NOTE: This is a general Read More
SDS-PAGE Protocol The basic protocol for Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) is commonly used to separate proteins according to their molecular weight. Materials Needed: Steps: This is a generalized protocol; parameters can differ based Read More
Gel electrophoresis Protocol Here is a basic protocol for agarose gel electrophoresis, often used for separating DNA fragments: Materials Needed: Steps: Always maintain safety procedures when dealing with ethidium bromide and UV light. They are Read More
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