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How are 5-ROX and 6-ROX dyes different from one another?

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In E. coli prokaryotic expression vector pET32a, does the TrxA tag lead to non-specific binding in WB and ELISA assays?

9th December 2023

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How are 5-ROX and 6-ROX dyes different from one another?
In my co-immunoprecipitation research, how can I set up a control?
Is it OK to use recombinant Human Macrophage Migration Inhibitory Factor (MIF) on the RAW264.7 mouse macrophage cell line?
Would it be possible to kill bacteria without damaging their protein structure?
In E. coli prokaryotic expression vector pET32a, does the TrxA tag lead to non-specific binding in WB and ELISA assays?

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How are 5-ROX and 6-ROX dyes different from one another?
In my co-immunoprecipitation research, how can I set up a control?
Is it OK to use recombinant Human Macrophage Migration Inhibitory Factor (MIF) on the RAW264.7 mouse macrophage cell line?
Would it be possible to kill bacteria without damaging their protein structure?
In E. coli prokaryotic expression vector pET32a, does the TrxA tag lead to non-specific binding in WB and ELISA assays?

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