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      Home Blog Page 173

      National Outstanding Youth Team of the University of Science and Technology of China is recruiting postdoctoral fellows in synthetic chemistry/energy materials chemistry with high salary (starting at 500,000 yuan)

      The National Outstanding Youth Team of the University of Science and Technology of China is recruiting postdoctoral fellows in the field of synthetic chemistry/energy materials chemistry with high salary (starting at 500,000 yuan)

      Professor Du Pingwu’s research group at the University of Science and Technology of China is looking for high-paying postdoctoral fellows in synthetic chemistry (organic chemistry, coordination chemistry, and inorganic synthesis) and energy chemistry (especially in urgent need of PhDs in organic synthesis, energy catalysis, electrocatalysis, and photocatalysis). To contact us, please send an email ( dupingwu@ustc.edu.cn ). Interested friends who love scientific research are welcome to join!

      1. Basic requirements for postdoctoral applications:
      1. Have political solid moral character, love higher education, abide by laws and regulations, be physically and mentally healthy, and have good conduct;
      2. Obtain a doctoral degree (both fresh graduates and previous graduates are welcome, and the previous graduation should not be more than 3 years ago);
      3. Be under 35 years old;
      4. Have specific proficiency in foreign languages ​​and computers, have independent scientific research and innovation capabilities, and be able to engage in postdoctoral research full-time.
      5. Publish 1 or more academic papers as the first author in the second-zone journals recognized by the Chinese Academy of Sciences.

      2. Postdoctoral entry situation and specific salary situation
      Postdoctoral entry unit: University of Science and Technology of China; location: Hefei City. This type of postdoctoral fellow is jointly trained with the Dongguan Institute of Technology. Training location: Professor Du Pingwu’s research group at China’s University of Science and Technology. Postdocs who meet the basic requirements will receive a pre-tax, comprehensive annual salary starting at 500,000 yuan. If you can also apply for the “Mozi Young Scholars” program of the University of Science and Technology of China and the National Bo Xin Talent Program, your annual salary can be further stacked!

      3. Fund application: Postdoctoral fellows and specially-appointed associate professors are encouraged to apply for national, provincial, and municipal funding projects. Postdoctoral fellows can apply for the China Postdoctoral Science Fund (80,000–180,000 yuan for natural sciences) after entering the station and the “Mozi Young Scholars” of the University of Science and Technology of China. Program, National Boxin Talent Plan, etc., and those who have received funding from the China Postdoctoral Science Foundation or key postdoctoral funding projects at or above the provincial level will be provided with matching funding by the Dongguan Municipal Finance at a ratio of 1:1. Distinguished associate professors can support applications for various national funds.

      4. Research directions of the research group:

      1. Research on energy catalysis (electrocatalysis, photocatalysis, and photoelectrocatalysis)

      2. Research on the synthesis and application of organically conjugated optoelectronic materials.

      We welcome those with experience in synthetic chemistry (both organic and inorganic synthesis are acceptable) and organic chemistry; young talents with backgrounds in inorganic chemistry, energy chemistry, etc., join! Those with experience in synthetic chemistry are preferred.

      5. Materials provided
      1. Personal resume (including study resume, work resume, published papers, project participation, etc.).
      2. Representative works, papers, and other relevant materials that prove your research ability and level.

      6. Contact information
      Professor Du Pingwu’s personal profile:
      Research group homepage: http://staff.ustc.edu.cn/~dupingwu/publications.html
      Contact person: Professor Du Pingwu
      e-mail: dupingwu@ustc.edu.cn

      7. Cooperation Instructor introduction:
      Du Pingwu, professor and doctoral supervisor at the University of Science and Technology of China, was selected for the National Outstanding Youth Fund, the National Youth Talent Program, and the New Century Excellent Talents Program of the Ministry of Education. He is currently a professor at the School of Chemistry and Materials Science at the University of Science and Technology of China, Hefei Microscale. He is a dual-appointed professor at the National Laboratory of Physical Sciences. He has long been engaged in the research of conjugated organic materials and optoelectronic functional materials. More than 170 academic papers were published in important international journals such as Acc. Chem. res., nat. commun., j. am. chem. soc., angew. chem. int. ed., adv. mater., etc., which others have cited. More than 13,000 times, h-index 67. Awards and honours received include Thieme Chemistry Journals Award (2018), the American Chemical Society Nobel Signature Award (2011), the American Chemical Society Young Scientist Award (Inorganic Chemistry) (2009), the National Scholarship for Outstanding Self-financed Students Abroad (2009), etc.

      8. Current situation of recent graduates in academia:

      Professor Han Ali/researcher, Chinese Academy of Sciences Hundred Talents Program (selective support), Shenyang Institute of Metal Research,
      Professor Ma Hao, University of Science and Technology of China, overseas, outstanding youth support,
      Professor Xu Peng, Chongqing University of Science and Technology,
      Associate Professor Lu Dapeng, Anhui Medical University,
      Jiang Daozhuan Associate Professor, Anhui University
      Associate Professor Sun Zijun Guangxi University of Science and Technology
      Associate Professor Cui Shengsheng, Shanghai Jiao Tong University
      Yan Zhiping Nanjing University of Posts and Telecommunications
      Associate Professor Jia Hongxing, Chongqing University
      . . .
      Many overseas postdoctoral fellows

       

      Institute of Laser Micro-Nano Manufacturing, School of Mechanical and Vehicle Engineering, Beijing Institute of Technology is recruiting postdoctoral fellows

      Team introduction:
      The Laser Micro-Nano Manufacturing Institute of the School of Mechanical and Vehicle Engineering of Beijing Institute of Technology currently has 1 academician of the Chinese Academy of Sciences and 4 national high-level leading talents, including the first batch of national scientific and technological innovation leaders/national outstanding youth/973 chief/863 subject experts / 1 member of the Science and Technology Commission of the Ministry of Education, 1 National Distinguished Young Scholar; 8 national high-level young talents, 4 associate professors/associate researchers, and 3 scientific research/administrative assistants. The academic leader took the lead in writing the national medium and long-term science and technology development plan in laser manufacturing and other directions, as well as 12 five-year field plans of the Ministry of Science and Technology, the National Science Foundation, and JKW. As the leader of the expert group, he took the lead in demonstrating and approving the first national key R&D plan in the manufacturing field. The key project “Additive Manufacturing and Laser Manufacturing” has received rolling support, and he serves as an editorial board member or deputy editor of 11 journals including “International Journal of Extreme Manufacturing”. Selected as Springer Professor (Honorary Distinguished Professor), OSA Fellow, ASME Fellow, and ISNM Fellow of the University of California, Berkeley.
      The institute is mainly engaged in research in the field of femtosecond laser manufacturing, covering theoretical modeling, online observation, processing methods, process equipment and its major engineering applications. Take the lead in undertaking the 973 projects of the Ministry of Science and Technology, national key R&D plan projects/topics, JKW foundation strengthening key projects/topics, national major science and technology special projects, National Foundation Commission major research plan integration/key projects, National Foundation Commission joint fund key projects, national outstanding projects Youth projects, etc. It has solved the theoretical and observational problems that have long restricted femtosecond laser manufacturing, successfully predicted a series of important anomalous effects, and has been confirmed by extensive international experiments. Proposed and implemented a new manufacturing principle of femtosecond laser Electronics Dynamics Control (EDC), realized active control at the electronic level in laser manufacturing for the first time, and expanded the ultimate capability of laser manufacturing. He invented a series of new EDC manufacturing technologies and equipment, and took the lead in realizing major engineering applications and industrial applications of femtosecond laser manufacturing. He took the lead in winning the second prize of the National Natural Science Award in 2016, the Ho Leung Ho Lee Science and Technology Innovation Award in 2017, the first prize of the Ministry of Education’s Technological Invention Award in 2018, the first prize of the Guangdong Provincial Science and Technology Progress Award in 2022, the National Innovation Award in 2023, etc.
      Job Requirements
      1) Have good character, be down-to-earth, hard-working, positive and optimistic, and rigorous in scholarship;
      2) The applicant has obtained or is about to obtain a doctorate;
      3) Have experience in (ultrafast) laser processing, optics, light-matter interaction, and semiconductor packaging Priority will be given to those with a certain background in
      scientific research and innovation; 4) Have a certain spirit of scientific research innovation and independent scientific research capabilities, a high sense of responsibility, and strong teamwork spirit and communication skills.
      Job Responsibilities
      1) Participate in the project research of the research group, have a solid academic background and a certain degree of free exploration ability;
      2) Participate in laboratory construction and daily management;
      3) Actively apply for various fund projects;
      Job benefits
      1) Special postdoctoral fellow The annual salary is RMB 300,000-500,000+; the annual salary for postdoctoral fellows in team positions is RMB 250,000+; the annual salary for postdoctoral fellows in scientific research positions is RMB 180,000+. Those selected for the postdoctoral innovative talent support program and the international exchange program introduction project will receive a supporting subsidy of 200,000 yuan/year, with a maximum annual salary of 700,000 yuan;
      2) Additional rewards will be received based on the academic achievements achieved (papers/patents/fund applications, etc.);
      3 ) Encourage and support applications for various postdoctoral programs. Actively support and encourage applications for various talent projects, fund grants, and domestic and foreign exchange opportunities.
      4) Social security and five social insurances and one fund;
      5) Postdoctoral apartments can be applied for during the station, and housing subsidies will be provided to postdocs who have not arranged an apartment;
      6) According to relevant policies, postdocs who stay in Beijing to work after leaving the station can apply for Beijing household registration for themselves and their spouses .
      For details, please see https://renshichu.bit.edu.cn/gb2bsh/index.htm

      Introduction to the co-supervisor:
      Zhang Xueqiang, an overseas high-level young talent, professor, and doctoral supervisor. PhD from the University of Notre Dame, USA, and conducted postdoctoral research at the University of Illinois at Urbana-Champaign and the University of California, Berkeley. Research contents mainly include femtosecond laser micro-nano processing, high spatial and temporal resolution imaging, and spectral observation, and explore the influence of ultrafast laser-material interaction mechanisms on material formation properties. Published 47 SCI papers, including (a total of) 30 first/corresponding author papers. Hosted overseas high-level talent youth projects, Natural Science Foundation Youth Fund, Ministry of Science and Technology Key R&D Plan Topics, JKW Key Project Topics, Aerospace Science and Industry Corporation Aerospace Science Fund, JKW Langyue Plan Pre-research Topics, ** Laboratory Innovation Fund, Huawei Horizontal topics of technology groups, etc. Young editorial board member of China Laser’s “Frontier Laser Manufacturing” sub-journal, Infomat, infoscience and Frontiers in Catalysis.

      Application/Contact Information
      We will provide a first-class scientific research platform and rich academic resources to help you become an outstanding researcher. If you are interested in joining the Institute of Laser Micro-Nano Manufacturing of BIT, please send your resume to the following email: xueqiangzhangme@bit.edu.cn

      Is there a way to determine which Abcam or Cell Signaling antibodies target which splice variant of gene X?

      Is there a way to determine which Abcam or Cell Signaling antibodies target which splice variant of gene X?

      Using antibodies bought from vendors like Cell Signaling or Abcam, you may get the following epitope information for a particular splice variant of gene X:

      1. Check Out Their Website: Check out the website of the antibody vendor, such as Cell Signaling or Abcam.
      2. Find an antibody: Type the name of the particular splice variant of gene X that you’re looking for into the search field.
      3. Third, Pick an Antibody: Go to the antibody’s product page from the search results.
      4. Locate Epitope Data: If the data is accessible, you may generally find it in the product image’s ‘Product Details’ or ‘Technical Information’ sections. This data can include the epitope, specific area, or sequence the antibody recognizes.

      If you are unable to locate the data:

      1. Reach Out to Support: In most cases, the company’s scientific support staff may be contacted if the epitope information cannot be found on the product website. If you have any queries or concerns, you can contact providers like Abcam or Cell Signaling using their contact forms, direct email, or phone lines.

      Important note: Due to proprietary constraints or a lack of mapping, epitope information is not always publicly accessible. If this is the case, the business may only be able to tell you if the antibody recognizes the right protein area and not the exact epitope sequence. Furthermore, product details are very antibody-specific. Polyclonal antibodies may identify several epitopes, unlike monoclonal antibodies, which typically have a fixed epitope.

      Remember that different gene isoforms and splice variants may react differently to antibodies; as a result, you may need to experiment with other antibodies or contact the manufacturer for more tailored guidance.

      How can benzocaine topical anaesthetic pain reliever spray be carcinogenic?

      How can benzocaine topical anaesthetic pain reliever spray be carcinogenic?

      Analgesics like benzocaine may be applied topically to various areas of the body to alleviate pain and other unpleasant sensations. One way it helps reduce pain is by inhibiting signals the nervous system uses to transmit pain.

      At this time, there is no conclusive evidence that benzocaine exposure causes cancer. On the other hand, you bring up benzene, a known carcinogen. Although they share a name, benzocaine and benzene are rather distinct chemicals. Here, worries about a possible cancer connection may stem from the fact that benzocaine can degrade into aniline, a chemical that has been associated with cancer in rare instances.

      The lack of a definitive cause-and-effect relationship between benzocaine and cancer in people should be emphasized once again. The medical usage and patient safety recommendations will be updated if new research provides more information on these possible hazards. Always check with your doctor for any questions or concerns before starting any new drug.

      What are the symptoms of benzene exposure and cancer potential?

      The United States Department of Health and Human Services and the International Agency for Research on Cancer (IARC) have both classified benzene as a carcinogen, indicating that it has the potential to cause cancer. Leukaemia and other malignancies of the blood cells are closely associated with it. Regarding benzene exposure and cancer, the following symptoms may manifest:

      Benzene Exposure Signs and Symptoms:

      1. Dizziness
      2. Rapid or irregular heartbeat
      3. Headaches
      4. Tremors
      5. Confusion
      6. Unconsciousness
      7. Death (at very high levels)

      Symptoms of long-term exposure include: 

      1. Anemia
      2. Decreased platelet count
      3. Blood abnormalities
      4. Damage to the immune system

      Signs of malignancies linked to benzene, such as leukaemia:

      1. Chronic fatigue or anaemia
      2. Unexplained bruising
      3. Weight loss
      4. Persistent infections
      5. Bone pain and tenderness
      6. Enlarged liver or spleen
      7. Frequent or severe nosebleeds

      Remember that benzene is usually dangerous in specific workplaces or for those dealing with gasoline and similar goods. The average person’s exposure is often far lower. Seek advice from a medical professional on the hazards of benzene exposure and how to avoid developing cancer if you are concerned about this.

      How are 5-ROX and 6-ROX dyes different from one another?

      How are 5-ROX and 6-ROX dyes different from one another?

      The ROX dye is connected to the nucleic acid molecule at spots 5 and 6, which are shown by the “5” and “6” prefixes. The dyes’ chemical characteristics and practical uses are similar; however, the dyes may have different fluorescence qualities.

      5-ROX: This dye, which is usually attached to the 5′ end of an oligonucleotide probe, gives off a steady fluorescent signal that might help make the changing fluorescence between wells more consistent.

      6-ROX: In a similar vein, this dye is often included in the oligonucleotide at the 6′ position. In addition to its normalizing value in fluorescence-based approaches, 6-ROX produces a fluorescent signal.

      The two dyes’ distinct peak wavelengths in their emission spectra serve as a primary identifier. It is vital to check the parameters of the detection equipment and dyes to get the best results since some may be better adapted for one dye over the other despite their seeming resemblance.

      Passive reference dyes, like ROX, are used to make sure that the fluorescence signal stays the same and does not change for reasons that are not related to the polymerase chain reaction. TaqManTM, Molecular BeaconsTM, ScorpionsTM, and FRET probes are all viable options for most qPCR applications.

      In my co-immunoprecipitation research, how can I set up a control?

      In my co-immunoprecipitation research, how can I set up a control?

      Controls are essential to ensuring the specificity and validity of your co-immunoprecipitation experiment. Several forms of regulation are listed below.

      1. IgG controls are negative controls; you should add an IgG control for non-specific binding. An IgG secondary antibody should not recognize any of the components in your lysate but should be of the same isotype and species as your primary antibody.

      Controlled Absence of Antibodies: To test for non-specific protein binding to the beads or non-specific precipitation, continue with the co-immunoprecipitation technique as usual, but leave out the primary antibody.

      2. Use a set of proteins already known to interact as a positive control, if at all feasible. In this way, the efficiency of your co-IP protocol may be confirmed.

      Load regulation (or input regulation): A fraction (typically 5–10 percent) of the cell lysate from your immunoprecipitation studies is put onto the gel to indicate that the target protein was present in the original sample.

      3. Thirdly, pre-clearing lysates are used as test controls. Agarose bead binding is a step that is done before co-IP to get rid of any proteins in the samples that do not specifically bind to the beads. The noise level in co-IP studies may be decreased with the use of this parameter.

      The immunoprecipitated protein western blot test is done to make sure that the target protein was successfully immunoprecipitated after the co-IP proteins have been separated on a gel.

      Consider the specifics of your experimental design and goals when deciding which controls to use. The highest level of scientific rigor and confidence in your findings depends on including these crucial controls.

      Is it OK to use recombinant Human Macrophage Migration Inhibitory Factor (MIF) on the RAW264.7 mouse macrophage cell line?

      Is it OK to use recombinant Human Macrophage Migration Inhibitory Factor (MIF) on the RAW264.7 mouse macrophage cell line?

      A changed form of human MIF can be used to treat the RAW 264.7 murine macrophage cell line that stops macrophages from migrating. Assuming that human MIF might interact with mouse cells, the main goal of this type of therapy is to see what kinds of responses or reactions mouse cells might have to human proteins.

      But there are a few things to keep in mind:

      Specificity to Species: (1) While many mammalian proteins have significant similarities across species, it’s always vital to consider species specificity. Although there is substantial sequence homology between human and mouse MIF proteins, and their activities are comparable, there may be bioactivity or receptor binding changes.

      The human MIF must attach to the receptors of its murine analog to have an effect. The reaction might be reduced or nonexistent if the human MIF has trouble binding to the mouse receptors.

      Experimental controls: Make sure that your experiments have valid control groups. It is best to compare cells treated with human recombinant MIF to cells treated with mouse recombinant MIF as a positive control group and cells that have not been treated as a negative control group.

      Given the circumstances, reading the relevant literature and designing your experiment with these considerations in mind is essential. You might also talk to a biology professor or specialist at your school or lab.

      Would it be possible to kill bacteria without damaging their protein structure?

      Would it be possible to kill bacteria without damaging their protein structure?

      There are several ways to kill bacteria without damaging protein structure or function. For example, consider the following frequent ones:

      1. First, heat inactivation: Although heat may be used to destroy bacteria, it can also denature proteins if it is applied at too high a temperature for too long. However, some bacteria may be killed by heating them to levels that do not denature their proteins. Although this approach is practical, it is challenging because the temperature and time needed for inactivation might differ across bacterial species.

      2. Chemical Fixation: Fixatives like formaldehyde and glutaraldehyde may cross-link proteins in bacteria, rendering them inactive. However, these chemical fixatives may also affect protein structure and function, depending on the chemical nature of the fixative and the protein of interest. To keep the balance between fixing bacteria and breaking down proteins, the concentration and length of time that they are exposed to these fixatives must be carefully controlled.

      3. Antibiotics: Antibiotic use is a reliable way to stop the growth of bacteria. Inhibiting bacterial development in this way does not lead to protein lysis or denaturation, but it may halt the spread of the bacteria. However, this may not be the best approach if eradication is necessary.

      4. Fourth, UV Irradiation: UV radiation, especially UV-C, may be utilized to inactivate bacteria. The approach is practical because it alters the DNA of the bacterium. However, this has the potential to harm proteins, particularly those that are vulnerable to UV radiation.

      5. Lastly, we have gamma irradiation, which uses high-energy gamma-ray or electron beam irradiation to successfully sterilize bacterial cultures without adding heat. Keeping the protein’s structure intact while sterilizing would be a good option, but it might be difficult to find the right equipment.

      Remember that no technique is perfect; your results may vary depending on the bacteria and proteins of interest. Various trials and adjustments might be required before settling on the optimal strategy.

      In E. coli prokaryotic expression vector pET32a, does the TrxA tag lead to non-specific binding in WB and ELISA assays?

      In E. coli prokaryotic expression vector pET32a, does the TrxA tag lead to non-specific binding in WB and ELISA assays?

      A TrxA tag is often used to make sure that recombinant proteins fold and dissolve correctly in E. coli expression systems and other studies that use protein expression. Because this tag is not found naturally in human cells, Western blotting (WB) and enzyme-linked immunosorbent assays (ELISAs) might attach it to the wrong cells.

      Non-specific binding may occur depending on several circumstances, but this is not guaranteed. The essential details are as follows:

      These are called primary and secondary antibodies. If you use non-specific primary or secondary antibodies in Western blotting or enzyme-linked immunosorbent assays (ELISA), they may bind to the TrxA tag or other background proteins instead of the protein of interest. The end effect may be deceiving because of this. If you want to lessen this possibility, you should use verified, highly specific antibodies against your target protein. Non-specific binding could be changed by the type and amount of blocking solution used in WB or ELISA. Non-specific binding might be reduced by testing various blocking agents.

      How to Clean: Non-specific binding may be minimized by using appropriate washing procedures to eliminate unbound or weakly bound antibodies.

      The Level of Antibody Prevalence: Non-specific binding may occur if too many antibodies exist. Maximize the efficiency of your antibody dilution so that the smallest quantity required for protein detection is used.

      General Bead or Plate Locations: If the reactive areas on the ELISA plate are not blocked, non-specific binding might occur. To avoid this from happening, make sure your blocking step is optimized.

      Remember, each new protein and tag combination can need tuning to decrease non-specific binding in your WB or ELISA. It’s crucial to validate and control each experiment individually to prevent false findings.

      How do I perform a restriction digest on total DNA isolation from mammalian cells?

      How do I perform a restriction digest on total DNA isolation from mammalian cells?

      Isolating whole DNA from mammalian cells and performing a restriction digest is a multi-step process. It is important to note that the specific experimental design and laboratory methods may need minor adjustments to this general technique.

      Here’s a high-level framework for thinking about it:

      Materials

      Isolated Genomic DNA

      Inhibitory Enzymes

      buffer for reactions

      “De-ionized” water

      Sterile pipette tips

      A set of six microcentrifuge tubes

      microcentrifuge

      Equipment for performing an agarose gel electrophoresis.

      A hot water bath or a heat block

      Procedure

      1. First, you’ll need to isolate total DNA from mammalian cells using either a phenol-chloroform extraction or a DNA isolation kit purchased from a store.

      2. After DNA purification, use a spectrophotometer or fluorometer to determine the DNA concentration.

      3 Get the response for restricted digestion going. For this, mix the following: There should be one to five milligrams of DNA, one to five units of restriction enzyme per milligram of DNA (as suggested by the manufacturer of the restriction enzyme), one-tenth of a liter of 1x restriction buffer (usually supplied by the manufacturer of the restriction enzyme), and enough deionized water to make the total volume to the best working volume (20 to 50 ul).

      4 Allow the reaction mixture to incubate at the temperature specified by the restriction enzyme’s manufacturer (typically 37°C). Depending on the restriction enzyme and the methods being followed in the lab, the incubation duration might be anywhere from 1 to 4 hours.

      5. Heat-inactivate the enzymes at 65–80 degrees Celsius for 20 minutes after incubation (depending on the specific enzyme used).

      Apply gel electrophoresis to your restriction digest for analysis.

      Always refer to the manufacturer’s instructions for the restriction enzymes you use to determine the optimal reaction conditions (concentration, temperature, and incubation time). Avoid contamination at all costs by strictly adhering to aseptic procedures.

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