Middle Tennessee State University (Murfreesboro, TN)
Read more about his post…
Credits: Source
Disclaimer
Academic & Faculty Support Specialist – Open Positions
Scientists Unveil Cheaper and Faster Way To Extract Lithium From Massive Untouched Reserves – Science News
A new solvent-based technique could change how lithium is extracted from brines, potentially making the process faster, cheaper, and viable in places where conventional methods fail. Few elements are as key to the clean energy transition as lithium, and global demand for it is soaring. The metal powers the rechargeable batteries inside electric vehicles and […]
Read more about this post…
Credits: Source
Disclaimer
Quantification Revisited: What qPCR Efficiency Models Reveal About Data Analysis Integrity – Research
Amplification efficiency is one of the key parameters in quantitative real-time PCR, as it directly influences the accuracy of both absolute and relative quantification. Amplification efficiency, the fold increase per cycle, is affected by oligonucleotide design, reaction chemistry, sample and template properties, and instrument performance. Consequently, it differs between samples, assays and experimental runs. Although methods for estimating the amplification efficiency have been available for more than two decades, most published qPCR studies continue to assume equal and ideal efficiency across assays. This simplifying assumption introduces efficiency- and expression-dependent error into relative expression and fold-change analyses, contributing to the poor reproducibility observed in many PCR-based studies. This review examines the role of the amplification efficiency in qPCR quantification; the consequences of ignoring, assuming or misapplying efficiency; and the practical implications for data interpretation. The review also considers the development of efficiency estimation models and their implications for contemporary analytical approaches, including emerging data-driven methods for amplification curve analyses.
Keywords:
PCR efficiency; data integrity; measurement uncertainty; qPCR; quantification; reproducibility; standard curve; ΔΔCq.
Read more about this post…
Credits: Source
Disclaimer
Harvard Engineers Build Chip That Twists Light To Reveal Its Hidden “Handedness” – Science News
A twisted pair of photonic crystals integrated with MEMS can dynamically control the handedness of light on a chip. Researchers at the Harvard John A. Paulson School of Engineering and Applied Sciences (SEAS) have developed a chip scale device that can actively control the “handedness” of light as it moves through it, a property known […]
Read more about this post…
Credits: Source
Disclaimer
Quantification Revisited: What qPCR Efficiency Models Reveal About Data Analysis Integrity – Research
Amplification efficiency is one of the key parameters in quantitative real-time PCR, as it directly influences the accuracy of both absolute and relative quantification. Amplification efficiency, the fold increase per cycle, is affected by oligonucleotide design, reaction chemistry, sample and template properties, and instrument performance. Consequently, it differs between samples, assays and experimental runs. Although methods for estimating the amplification efficiency have been available for more than two decades, most published qPCR studies continue to assume equal and ideal efficiency across assays. This simplifying assumption introduces efficiency- and expression-dependent error into relative expression and fold-change analyses, contributing to the poor reproducibility observed in many PCR-based studies. This review examines the role of the amplification efficiency in qPCR quantification; the consequences of ignoring, assuming or misapplying efficiency; and the practical implications for data interpretation. The review also considers the development of efficiency estimation models and their implications for contemporary analytical approaches, including emerging data-driven methods for amplification curve analyses.
Keywords:
PCR efficiency; data integrity; measurement uncertainty; qPCR; quantification; reproducibility; standard curve; ΔΔCq.
Read more about this post…
Credits: Source
Disclaimer
Scientists Just Found a Hidden Oxygen Drain Inside Plant Cells – Science News
Scientists discovered that mitochondria inside plant cells can pull oxygen away from chloroplasts, revealing a hidden mechanism that may help plants adapt to stress. A team of scientists at the University of Helsinki has discovered a previously unknown interaction inside plant cells. Their research shows that mitochondria can draw molecular oxygen away from chloroplasts. This […]
Read more about this post…
Credits: Source
Disclaimer
Beyond Epilepsy Control: Repurposing Antiepileptic Drugs in Central Nervous System Tumor Therapy – Research
Antiepileptic drugs (AEDs) are primarily indicated for controlling epileptic seizures. However, accumulating clinical evidence suggests that their benefits in patients with central nervous system (CNS) tumors extend beyond seizure management. Emerging evidence indicates that AEDs possess direct antitumor activity independent of their antiepileptic effects, highlighting a promising novel direction for CNS tumor therapy. This review elucidates the multifaceted antitumor mechanisms of classic (e.g., valproic acid and levetiracetam) and novel (e.g., cannabidiol) AEDs, including their impacts on metabolic reprogramming, epigenetic regulation, endoplasmic reticulum stress and unfolded protein response (ERS-UPR), ion homeostasis, and the tumor immune microenvironment (TIME) to provide new insights and a theoretical basis for developing multitarget therapeutic strategies.
Keywords:
antiepileptic drugs; central nervous system tumors; drug repurposing; endoplasmic reticulum stress; epigenetic regulation; ion homeostasis; metabolic reprogramming; tumor immune microenvironment.
Read more about this post…
Credits: Source
Disclaimer
Quantification Revisited: What qPCR Efficiency Models Reveal About Data Analysis Integrity – Research
Amplification efficiency is one of the key parameters in quantitative real-time PCR, as it directly influences the accuracy of both absolute and relative quantification. Amplification efficiency, the fold increase per cycle, is affected by oligonucleotide design, reaction chemistry, sample and template properties, and instrument performance. Consequently, it differs between samples, assays and experimental runs. Although methods for estimating the amplification efficiency have been available for more than two decades, most published qPCR studies continue to assume equal and ideal efficiency across assays. This simplifying assumption introduces efficiency- and expression-dependent error into relative expression and fold-change analyses, contributing to the poor reproducibility observed in many PCR-based studies. This review examines the role of the amplification efficiency in qPCR quantification; the consequences of ignoring, assuming or misapplying efficiency; and the practical implications for data interpretation. The review also considers the development of efficiency estimation models and their implications for contemporary analytical approaches, including emerging data-driven methods for amplification curve analyses.
Keywords:
PCR efficiency; data integrity; measurement uncertainty; qPCR; quantification; reproducibility; standard curve; ΔΔCq.
Read more about this post…
Credits: Source
Disclaimer
Scientists Solve 2,700-Year-Old Eclipse Mystery – and Uncover Evidence About the Sun’s Activity – Science News
An ancient Chinese eclipse record helped scientists refine Earth’s rotation data and confirm rising solar activity after a prolonged quiet phase. An international team combined historical geography with modern astronomical modeling to reassess what is considered the earliest precisely datable record of a total solar eclipse. By reconstructing how the Sun would have appeared from […]
Read more about this post…
Credits: Source
Disclaimer
Serving 