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In E. coli prokaryotic expression vector pET32a, does the TrxA tag lead to non-specific binding in WB and ELISA assays?

In E. coli prokaryotic expression vector pET32a, does the TrxA tag lead to non-specific binding in WB and ELISA assays? A TrxA tag is often...

How do I perform a restriction digest on total DNA isolation from mammalian cells?

How do I perform a restriction digest on total DNA isolation from mammalian cells? Isolating whole DNA from mammalian cells and performing a restriction digest...
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