Current CRISPR guide RNAs are designed based on European reference genomes, leading to false-negative results in cells from people with African ancestry.



Summary

Current CRISPR guide RNAs, crucial for precise gene editing, are primarily designed using European reference genomes. This presents a significant problem for individuals with African ancestry, who have greater genetic diversity not fully captured in these reference datasets. As a result, these guide RNAs may not perfectly match the target sequences in African-derived cells, leading to mismatches. These mismatches can cause the CRISPR system to fail to recognize and cut the intended DNA site, resulting in false-negative outcomes and potentially hindering accurate research and therapeutic applications for this population.

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