Tags :protocol

Protocols

Immunofluorescence Protocol (FFPE)

Immunofluorescence Protocol (FFPE) A general protocol for performing immunofluorescence on formalin-fixed paraffin-embedded (FFPE) samples. Materials: 1. FFPE tissue sections/slides 2. Primary and secondary antibodies 3. Xylene 4. Ethanol in various concentrations 5. Citrate buffer (pH 6.0) or similar antigen retrieval Read More

Protocols

Immunofluorescence Protocol

Immunofluorescence Protocol General protocol for immunofluorescence. Please ensure you understand all steps and safety guidelines well before you proceed. Materials: 1. Primary and secondary antibodies 2. Phosphate-buffered saline (PBS) 3. Blocking solution 4. PFA fixative 5. Detergent (Triton X-100) 6. Glass Read More

error: Alert: Content is protected !!