Ammonium Sulphate Fractionation of Antibodies (Crude Protein Purification) Abstract A “cut” with ammonium sulphate was the go-to procedure for isolating IgG and other blood proteins until Protein A (rabbit) and Protein G (rodent) were widely Read More
Akta Pure Protein Purification Protocol Protein purification is a sequence of processes intended to isolate a singular type of protein from a complex mixture. ÄKTA pure is an adaptable and flexible chromatography system for preparative Read More
Nickel Column Protein Purification Protocol Nickel affinity column chromatography, also known as immobilized metal affinity chromatography (IMAC), is a common method for purifying recombinant proteins that are engineered to have a hexahistidine tag (6xHis tag) Read More
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