SDS-PAGE Gel Preparation 1. Prepare the separation gel (10%). Mix in the following order: H2O 4.1 mL Acrylamide/bis (30% 37.5:1; Bio-Rad) 3.3 mL Tris-HCl (1.5 M, pH 8.8) 2.5 mL SDS, 10% 100 µL N,N,N′,N′-tetramethylethylene-diamine (TEMED) (Bio-Rad)  10 µL Ammonium Read More