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In E. coli prokaryotic expression vector pET32a, does the TrxA tag lead to non-specific binding in WB and ELISA assays?

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In E. coli prokaryotic expression vector pET32a, does the TrxA

Dr AF Saeed Dr AF Saeed 16th November 2023

In E. coli prokaryotic expression vector pET32a, does the TrxA tag lead to non-specific binding in WB and ELISA assays? A TrxA tag is often used to make sure that recombinant proteins fold and Read More

ELISA Immunoassay Detailed Protocol

ELISA Immunoassay Detailed Protocol

Dr AF Saeed Dr AF Saeed 28th August 2023

ELISA Immunoassay Detailed Protocol Enzyme-linked immunosorbent Assay (ELISA) is a biochemical method used to detect the presence and concentration of antibodies or antigens in a sample. Materials needed: – ELISA Plates – Target antigen or antibody – Non-reactive Read More

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