The College of Veterinary Medicine at Long Island University (LIU-CVM) (Post Campus) invites applications for a position of Postdoctoral Fellow.
LIU-CVM (https://liu.edu/Vetmed) is located in LIU Post campus and has an interdisciplinary research program with highly accomplished scientists and board-certified veterinary pathologists. The research program consists of basic, translational, and clinical research with combined expertise in cellular, molecular, genetic, and clinical sciences. Research focuses include infectious diseases, autoimmune diseases, cancers, and tissue regeneration. The goals of the research program are to improve the understanding of disease pathogeneses and to utilize the newly gained knowledge on disease pathogeneses to develop novel therapeutics. Newly renovated research laboratories within the LIU-CVM are equipped with state-of-the-art instruments for the above-mentioned research activities. The incumbent of this postdoctoral fellow position will participate in NIH-funded research project in the laboratory of immune regulation and stem cells (https://www.liu.edu/VetMed/Departments/~/link.aspx?_id=4A2A666EA916470BAD1D8949A3F610DC&_z=z). These research projects use cutting-edge technologies to investigate the mechanisms that control the regenerative capacity of intestinal stem cells as well as the mechanisms that regulate immune responses. Our ultimate goals are to develop novel therapeutic strategies for immune-mediated diseases such as inflammatory bowel disease and multiple sclerosis. Specific duties include but not limited to:
Setting up cell culture for primary cells and cell lines. Experience with culture of intestinal crypts is a plus.
Transducing cells with lentiviruses.
Performing assays to evaluate cell functions such as proliferation, cytokine secretion, apoptosis, and differentiation.
Performing various analyses using flow cytometer such as cell subset analysis, cell cycle analysis among others.
Isolating DNA and RNA.
Performing agarose gel electrophoresis, Cloning, PCR, real time RT-qPCR, ChIP, ChIP-Seq.
Isolating and quantifying proteins.
Performing SDS-PAGE, western blotting, immunoprecipitation, in vitro and in vivo biochemical assays.
Preparing paraffin-embedded and/or cryosections.
Performing histology, H&E staining, and immunohistochemistry staining
Analyzing images using bright field, fluorescence, and confocal microscopes.
Using UV-Vis spectroscopy and spectrophotometry to quantify DNA, RNA, and protein.
Performing sequence alignment.
Analyzing ChIP-seq and RNA-Seq data.
Small Animal Handling
Breeding and maintaining mouse colonies.
Genotyping for knockout and transgenic mouse models.
Performing animal procedures such as isolation of intestinal crypts, intravenous/intraperitoneal/subcutaneous injections, necropsy and tissue collection, immunohistochemistry of tissue sections.
Performing luciferase imaging of live animals.
Mentoring undergraduate, Doctor of Veterinary Medicine student, Master, and PhD students for immunology, stem cell biology, and basic laboratory techniques.
Gathering and interpreting experimental data, preparing reports and manuscripts.
Performing other duties as assigned.
The Successful Applicant:
Will have at least one year of experience directly related to the above assigned duties.
Will demonstrate excellent organization skills.
Willing to work on multiple projects and able to work effectively with other laboratory members.
Willing to work effectively with other faculty, staff, and administrators as necessary.
Will demonstrate the proven ability to independently carry out major projects of importance to the University and the community.
Will have a strong and consistent publication record in the field of tissue repair and/or immunology.
Minimal qualifications include a PhD in natural science, or a health-related field from a college or university of recognized standing.
Cover letter, resume, transcripts if recently graduated, previous research accomplishment, and future research plan.
LIU is an equal opportunity employer. LIU is committed to extending equal opportunity in employment to all qualified candidates who can contribute to the diversity and excellence of our academic community. LIU encourages applications from all qualified individuals without regard to race, color, religion, genetic information, sexual orientation, gender and/or gender identity or expression, marital or parental status, national origin, ethnicity, citizenship status, veteran or military status, age, disability or any other basis protected by applicable local, state or federal laws. Hiring is contingent on eligibility to work in the United States.